Determination of Optimal Non-Denaturing Elution Conditions from Affinity Columns by a Solid-Phase Screen
نویسندگان
چکیده
منابع مشابه
Biospecific Elution from Non-Specific Adsorbents (‘Affinity Elution’)
Enzymes adsorbed to ion exchangers are in many cases specifically desorbed by interaction with their ligands. This phenomenon has been variously called ‘specific elution with substrate’ or ‘affinity elution’ in the literature (von der Haar, 1974). Because the most effective ligands for elution are those containing charged groups identical with those on the ion exchanger (positive groups in the ...
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Fibronectin was purified from human plasma by affinity chromatography under nondenaturing conditions. The method was based on the previously known binding of fibronectin to gelatin. The novel features of our method are the use of arginine in the elution of fibronectin from immobilized gelatin [Vuento & Vaheri (1978) Biochem. J. 175, 333-336] and the use of arginine-agarose as second affinity st...
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Solid-phase extraction methods are being used for increasing numbers of applications in lipid analysis, but especially as a rapid means of isolating particular components for further analysis. Many different types of packing material are available enabling most different modes of chromatography to be used, including adsorption, reversed-phase, silver-ion and ion-exchange. However, there are occ...
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A simple, rapid and sensitive solvent assisted-dispersive solid phase extraction method was developed for the extraction of iron(II) prior to its spectrophotometric determination. The Fe(II) reacted with 2,4,6-tris(2-pyridyl)-1,3,5-triazine, neutralized with sodium dodecyl sulfate and extracted onto the fine particles of benzophenone which were formed upon rapid injection of a mixture of benzop...
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The affinity chromatographic separation procedure was tested for its utility in quantitating the affinity of proteins to both insolubilized ligand and corresponding competing soluble ligand. An expression has been derived that (1) allows the determination of binding constants for the interaction of a soluble protein species with an affinity chromatography matrix involving active site binding, (...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2001
ISSN: 0736-6205,1940-9818
DOI: 10.2144/01313rr03